3D Mesenchymal Stem Cell Culturing Systems
– Extracellular vesicles (EVs) derived from 3D mesenchymal stem cell (MSC) cultures offer a significant advantage over traditional 2D systems due to their higher yield, enhanced bioactivity, and therapeutic potency. The 3D culture environment closely replicates native tissue conditions, leading to improved EV production, optimized cargo composition (e.g., enriched proteins and RNAs), and superior regenerative and immunomodulatory effects.
Isolation of Mesenchymal Stem Cells from Whole Blood Cells
– Isolating mesenchymal stem cells (MSCs) directly from whole blood is a complex but feasible process. MSCs are multipotent stem cells typically isolated from bone marrow, adipose tissue, or other sources. While blood is not a primary source of MSCs, some protocols allow for their isolation, leveraging their rare presence in the blood under certain conditions, such as following tissue injury or specific mobilization treatments.
